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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical job in selling cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two more splice variants have been reported but thorough nucleotide sequence information isn't still obtainable.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This tutorial outlines more probable optimization of mobile and immunoassay parameters to guarantee the best possible success are...
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This tutorial outlines additional probable optimization of mobile and immunoassay parameters to ensure the very best outcomes are...
Antibodies sure to the blocking peptide no longer bind on the epitope to the concentrate on protein. By comparing the staining within the blocked antibody versus the antibody by yourself, you can see which staining is distinct.
Target Data Cyclin E belongs on the remarkably conserved cyclin family members, whose users are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation patterns which lead to your temporal coordination of each mitotic function. Cyclin E varieties a posh with and features as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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